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Hello everyone. This is Calimara here. And no, it's not Calamari. Welcome back
to my channel. Or if you're new to the pond, go ahead and take a dive. You
might like it here. So, this video is a bit different from the kind of content I
usually do, but recently I feel like I haven't really been able to get as much
content out as I used to, at least not regularly. And a lot of that has a lot
to do with what I have to do in real life now that I'm in medical school. And
it might sound silly, but even though I'm doing something that I've always
dreamed of doing and is something that I'm very passionate about, I still feel
guilty about not fulfilling my YouTube obligations, which is as equally
important to me. So, I thought maybe I could do a blend of both by making a
little vlog of what I've been actually up to all this time when I vanish off of
social media with no notice. You guys might know that I'm not the kind of
YouTuber who shares a lot of my life. I don't usually uh indulge a lot about
myself other than what is relevant for my redesigns and stories and stuff, but
you know, I figured I could take a small leap of faith. And here we are. So,
today we are at we are at microbiology lab. So, we're
going to be playing around with some bacteria, and we're going to fill in
this fun little worksheet here to capture the kinds of bacteria we managed
to stain. And this is what we're going to be doing. Basically, uh my first time
staining bacteria. Exciting. And who says medicine and art don't mix? And now
we're just walking into the microbiology lab. We don't always get to bring our
phones. Usually only certain labs allow it. And this is my friend Fica. By the
way, usually only labs where we have to take photos and draw allow us to take
our phones in. And this is one of them. And here is our microbiology professor
telling us what strains of bacteria we're working with. And today we have
eststeria coli, streptococcus pneumonia, stafylocus orureas and a mix of
eststeria coli and stafylocus. And he also wastes no time in telling us
about how we're going to be examined for our practical exam. Basically, what
we're going to have to do is we're going to have to stain our own bacteria and
we're going to have to look at it under a microscope and we're going to have to
figure out what strain of bacteria it is. Yay. Love that for us. Okay.
Slide. And now our professor is showing us how it's done. So, first things
first, you got to prepare your dipping stick. And just don't forget to swirl
your bacteria just in case they've settled at the bottom. Prepare your
slide. And don't forget a beaker to dispose of the used dipping stick. You
have to be careful when opening the lid. I found that it's very firmly on there.
And I accidentally splashed myself with a bit of stafylocus,
but you know, it's all good. Just make sure you get a good amount of bacteria.
Don't be shy with your amount. And our professor said, "As long as you're still
using the same dipping stick, you can double dip as many times as you want."
All right, now that we have our bacteria on our slide, we can either air dry it,
but that takes too long. And we have fire, so that's what we're going to do.
So, just, you know, very quickly and carefully pass the slide over the fire.
Make sure not to hurt yourself doing this. And don't hold it too long over
the fire or it will break the glass. Now that our bacteria is properly fixated,
we get to stain. We're going to start off with some crystal violet, which our
professor has dropped onto the slide. And we're just going to swirl it around.
Make sure it has properly covered the surface area of where we swiped our
bacteria. And then we're going to leave this on for 1 minute or 60 seconds. So,
it's important that we keep a timer here. And when you're just a bystander
watching it, it feels like forever. But once you're actually over there holding
the slide, it flies by in a blink of an eye. Especially when you're just having
fun swirling the dye around. And once it's ready, what you got to do
here is just make sure you keep the water pressure low and pass it on very
carefully across the slide cuz you don't want to accidentally wash off the
bacteria you just fixated on there. And after that, we're going to go in with
some iodine, which we are also going to leave for another 60 seconds. So, just
drop it on. Same procedure as your crystal violet. And set your timer for
another minute. [Music]
All right, time to wash off the iodine. Once again, just very carefully rinse
it. You don't want to wipe off the bacteria, which is, I know,
counterintuitive usually when you're washing something. And now we finally
get to the second to last step, which is our alcohol, which we will use as our
decolorizer, just until the liquid runs clear to get rid of the excess iodine
and crystal violet and allow for the counter stain to stain later on. And
then you quickly wash it off because you don't want to accidentally destroy the
bacteria. And now comes the last step which is the saffranin which is our
counter stain. It'll fill the bacteria with a really nice cute pink color. And
unlike your crystal violet and iodine, you're only going to leave the saffran
on for 45 seconds before you rinse it off.
[Music] And congratulations, you've successfully
stained some bacteria. Now, all you got to do is leave your slide to air dry. No
buns and burner this time, and it'll be ready for you to observe under the
microscope with a bit of immersion oil. And here's my attempt at staining
bacteria. So, for the lab, we were divided into groups of four. And this is
my friend recording me. So let's see how I go. So for my bacteria, I'll be
staining the stafylocus aras, which is what I'll be splashed with later on. But
as you can see here, I think this is when it happens actually. Um, I'm just
preparing my dipstick. And I know what's coming. I don't know if it's noticeable
on camera though. But yeah, preparing my dipstick. And then I was saying to my
friend here that we should label this in case we want to double dip with it. And
they were all like, "Yeah, that's a good idea." So I take it out. I asked my
friend for one of the labels that we prepared so I can label my dipstick.
And then I'm going to open my cap here. And then it splashed on me. So I don't
think you see it, but it happened. Anyway, I put my dipstick in and then
when it's in there, I remember that I I didn't swirl it. So, I just I think that
was when it happened. I don't know. There there could have been multiple
points where I was splashed with it. And I I'll just be straight up with you.
This stain didn't go well. I think it was because I forgot to swirl the
bacteria. So, I might not have caught it on my dipstick cuz it was just blank. or
I don't know, maybe I rinsed it too hard, too. You'll see later on. It's It
was an attempt. An attempt was made, but you know, I tried my best. This is what
the dipsticks look like, by the way. It's not like a a little spoon. It's
actually um like a doughnut shaped. It's empty in the middle. And I guess you
just got to blow little bacteria book holes with it.
I tried to be generous with my bacteria amount cuz I wanted a a good chance that
I would get something, but as I've already told you, I didn't get any with
this attempt. But I do get it on my second attempt. So, I guess the key is
to not rinse too much and also to make sure you remember to swirl your vial cuz
I think that makes a big difference. And now we get a bit of a treat because
we get to commit arson. Yay.
So, this is actually my favorite part of gram staining bacteria is when you just
get to kind of wave the slide over the fire and just watch it dry. I don't
know, it's just really fun. The key here is to make sure that your slide is
completely dry cuz you don't want to accidentally have the bacteria sliding
around everywhere when you rinse it. And when you're done, just plop the lid on
there and move on with your day. For technical reasons, we were
instructed to do the staining over the sink. And this is probably something you
should keep in mind as well when you're using labs like this if you want to keep
everything nice and tidy, is to make sure that you don't just stain anywhere
you want willy-nilly. And make sure to wipe your bench down after every
bacterial swab so you don't contaminate your slides.
Now, on with the dying or staining, I guess I should say. Am I putting a
copious amount, an excessive amount on there? Yes. But also, I covered a very
large area of my slide with bacteria, and I just wanted to make sure that the
entire perimeter was stained. And we're just going to wait here for one minute,
which luckily you don't have to with the power of editing.
And there we are, ready for the iodine stain, which I will also be putting an
excessive amount of naturally. And once again, we're going to be
waiting for another minute. [Music]
So, I think this is potentially where it all went wrong for me. If you watch me
rinse it, you can see that I hold it under the water for just way too long. I
definitely shouldn't have done this. I definitely just completely washed away
all the bacteria I've fixed on there. But obviously, past Cali was completely
oblivious to this, so she's just going to keep trucking on with the
decolorizer. [Music]
And again, I think I definitely definitely rinsed it too much and I used
too strong of a water pressure on it. And honestly, watching this back, it
makes complete sense why my bacteria did not show up at all.
Well, sweet cookie cats. We'll always remember the time we spent together.
[Music] All right, pop quiz. How long do we
leave the Sephran on for? Okay, time's up. If you guessed 45
seconds, congrats. you would have scored one point on the practical exam. And
yeah, that's about it. That wraps up my catastrophic first attempt at
grandstaining bacteria. You can see here me trying to do it properly this time by
swirling the vial first. And the second attempt actually worked out. So, you
know, learn from my mistakes. Do as I say, not as I do. And this is me with
one of our dried slides ready for um examination under the microscope. I've
just dropped some immersion oil onto it so that I could actually
get high definition under the microscope there. Um I'm just spreading it around
like I did with the dye. And then I'm just plopping it onto our very nice
microscope here. And fun fact, I am actually wearing contacts uh that day.
And I don't know why I did that. I knew I had microbiology lab and I knew we
were going to be playing around with bacteria. And the thing is I have very
bad eyesight. So, um, I'm I'm nearsighted and therefore when I have to
look into the microscope, which already compensates for nearsightedness, I just
get a massive headache because my contacts and the microscope just don't
mix. Technically, you can adjust for the vision as well, but I did not think to
do that for some reason. So, I I've just had to deal with it. Um because,
you know, hindsight is 2020, but my vision is not. It also doesn't help that
I was probably looking at a blank slide here because I washed off all of my
bacteria. But luckily, one of our slides was
successfully stained. And this is what it looks like.
Pretty cool, right? So these are how our slides came out.
Technically there are four, but one of them is just a mix of two of these. But
essentially on the left over there you have our streptocous pneumonia. And you
can tell that it is streptocockus because of the way it's distributed. It
kind of looks stringy. And if you really squint, you can see that the bacteria is
shaped like a c circle. So they're round, which is how it got its name,
caucus. And technically, they are diploid bacteria, meaning they come in
pairs. They're not singular. But when they clump together like that, they tend
to form a chain, which is why they're called streptocockus, which strepto
basically means chain. And it just means that they are round and they form a
chain. And the these are the guys that will cause pneumonia.
As for the guy in the middle there, that is our stafylocus aras, the one that
splashed my lab coat. And we can tell that it is stafylocus because once
again, it is circular in shape. And this one actually forms grape clusters. So
they kind of look like a bunch of grapes like clumped together. And you can see
how different they are when they group together. They kind of tend to form more
like uh clumps whereas the strepto caucus forms like strings. And that's
how you can tell them apart. And finally, we have the most distinct
sample of them all, which is our eststeria coli, which is our gram
negative bacteria. So those other two, the purple guys, those are our gram
positives and the eststeria is our gram negative bacteria. And the reason that
it's pink is because technically our crystal violet dye can only stain the
tacoic acid in the cell wall which is part of the pepidoglycen layer which is
just it's a specific protein that gram positive bacteria have in their cell
wall cuz it has a thicker cell wall compared to our gram negative. Whereas
our gram negative has a thin cell wall which has a thin pepidoglyen layer but
also a layer of lipopolysaccharides over it which is just to say it's a
layer of it's like a sheer layer of fat on top which stops our crystal violet
from actually dying the pepidoglycin layer underneath it and that's why it
instead stains pink. Aside from the gram differentiation, you can also see that
it is shaped very distinctly unlike our two other samples which were balls.
Balls. Our esteria coli is shaped like a rod
and that's why it's also classified as a type of basilus bacteria. And now here I
am working on my little worksheet. I really wish I had recorded over my paper
so you could actually see the art process because this was in fact also a
speed draw because we had to handdraw what we saw in the microscope uh on our
slides. This is kind of similar to what we do for hisystologology class where we
would usually also draw what we saw on our slides and label it accordingly. Um
the difference though is that for our histologology class we are usually
allowed to do it digitally which of course you know I always do it digitally
but this was actually quite a nice change and I wish I was I had the
forethought to record overhead so that this video despite it being like a
medical school vlog could still have a speed draw. But you know you take what
you can get. This section specifically was just me answering some of the short
answer questions um on the report. And honestly, I'm surprised at how useful my
art skills have been for medical school, but not really the other way around. Cuz
I've actually gotten an interview question once about if my learning
anatomy helps me draw better. And I confidently and very certainly said no
because it really didn't. But art did actually help a lot in medical school,
especially with like envisioning spaces and like projecting where things might
be under the skin with organs and bones and such. So yeah, I don't know. I kind
of just feel like I'm really in my element and despite the long hours, I'm
really happy. And granted, not every day is as
exciting as this. We're not always doing super cool experiments and labs every
single day. Though, we do get a lot of practical experience in our curriculum.
Most of our time is spent in you know lecture halls learning the theory and
then doing a lot of case studies and just kind of a lot of research and
reading as well to give us that foundation for our practice moving
forward. But today was a particularly interesting day and I'm really glad that
I was able to get it on camera if if for nothing but my own amusement.
[Music] Once lab was done, I was finally able to
have lunch. Of course, I needed coffee. There was a fair going on, I think, like
um independent business fair happening that day, so I just bought whatever was
available from the stalls there. I got a salad wrap, which was pretty good. But
uh you'll see in a second when I open the wrap. The wrap was not in fact a
wrap, but it had come undone inside of this paper. And well, you know, I still
ate it. It was just a challenge to do so and a bit messy to do. So
don't worry, I cleaned that up. After lunch, we had our second lab of
the day, which is for pharmarmacology. For this lab, we were learning about
individual responses to medications, um, specifically dasipam as our test drug
today. And we've just done our tests and now we're learning how to calculate all
the different data sets we got. So, I didn't record much in this lab because
we were working with uh test animals, which I always kind of get a bit
emotional about cuz I don't know. I just I like them too much. But here they are
after their hard work. They're a bit sleepy. Great job today, guys. And after
all the labs, I still had one more class, which is a general subject that
is mandatory for everyone regardless of faculty to take. And that lasted for
another 2 hours before I was finally able to go home. I just got home.
Really tired. had a nice little Oh,
[Music] that's that's not very professional
beauty guru of me. I've actually taken most of my makeup off already, but I
forgot to record it. Still have one more cleanse left to do.
And then after this, I'll also wash my face with soap and water. I had really
bad skin when I was a teenager, and I don't know why. And also, that light
doesn't work. It never does cuz why wouldn't you know? I had really bad skin
as a teenager, right? Picking off where I left off. I think it was genetics or
maybe it was like chronic stress or something, but I had to go on Accutane
for it and now my skin is okay. Well, it's mostly cuz I use this cream now
that really helps. Um, not sponsored or anything, but like you
know, if you want to sponsor me, I use this one and it's so good. Literally,
this is the only product I've ever like I could ever say actually changed my
skin and made it better and like stopped me from breaking out constantly. Like
the moment I stopped using that, the spots come back in and it's kind of
scary. And this is the cleanser I use, you would have seen it already cuz it
was covering my face. Anyway, that's
that's my vlog. I don't really I haven't figured the whole thing out yet, but if
you're interested, I'm not going to say that. No, I'm not like other YouTubers.
Anyway, yeah, I'm just I'll just make more if I think I want to do more. So,
I will see you all in the next video. Goodbye.
[Music] 12 a.m.
I guess I'll just wait till you get home.
Oh. [Music]